Heterologous production of secondary metabolites as pharmaceuticals in Saccharomyces cerevisiae

  title={Heterologous production of secondary metabolites as pharmaceuticals in Saccharomyces cerevisiae},
  author={Beibei Huang and Jing Guo and Bo Yi and Xiaojing Yu and Lianna Sun and Wansheng Chen},
  journal={Biotechnology Letters},
Heterologous expression of genes involved in the biosynthesis of various products is of increasing interest in biotechnology and in drug research and development. Microbial cells are most appropriate for this purpose. Availability of more microbial genomic sequences in recent years has greatly facilitated the elucidation of metabolic and regulatory networks and helped gain overproduction of desired metabolites or create novel production of commercially important compounds. Saccharomyces… 
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The engineering of the baker’s yeast Saccharomyces cerevisiae to produce ergothioneine by fermentation in defined media provides a promising process for the biosynthetic production of ERG.
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The engineering of the baker's yeast Saccharomyces cerevisiae to produce ergothioneine by fermentation in defined media provides a promising process for the biosynthetic production of ERG.
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This study shows the significance of bioactive compounds produced by S. cerevisiae species with their possible activity and value in chemotherapeutic drugs pipeline and the isolation and alteration of these natural secondary metabolites would promote the development of chemotherAPEutic drugs.
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This work reports the engineering of Escherichia coli genes encoding the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway into the genome of Saccharomyces cerevisiae and the characterization of intermediate metabolites synthesized by the MEP pathway in yeast and shows that S. Cerevisiae has the capability to functionally express at least some bacterial iron–sulfur cluster proteins in its cytosol.


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This review will describe some of the efforts on using S. cerevisiae as a cell factory for the biosynthesis of high-value natural products that belong to the families of isoprenoids, flavonoids and long chain polyunsaturated fatty acids.
Metabolic pathway engineering for complex polyketide biosynthesis in Saccharomyces cerevisiae.
This work describes the introduction into S. cerevisiae of pathways for the production of methylmalonyl-coenzyme A (CoA), a precursor for complex polyketides, by both propionyl-CoA-dependent and propiony- CoA-independent routes, and demonstrates that the methylmalonies produced in the engineered yeast strains is used in vivo for theproduction of a polyketide product, a triketide lactone.
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Despite the prospect of obtaining major improvement through metabolic engineering, this approach is, however, not expected to completely replace the classical approach to strain improvement-random mutagenesis followed by screening.
Genetic engineering of taxol biosynthetic genes in Saccharomyces cerevisiae.
Genetic analysis showed that yeast isoprenoid precursors could be utilized in the reconstituted pathway because products accumulated from the first two engineered pathway steps (leading to the committed intermediate taxadiene); however, a pathway restriction was encountered at the first cytochrome P450 hydroxylation step.
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The potential use of Saccharomyces cerevisiae as an alternative engineering tool is investigated and it is shown that geraniol synthase expression affects the general ergosterol pathway, but in a manner dependent on the genetic background of the strain.
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In this chapter, the benefits of using metabolic engineering approaches for the development of green chemistry are illustrated by the recent advances in microbial production of isoprenoids, a diverse and important group of natural compounds with numerous existing and potential commercial applications.
Non-conventional yeasts as hosts for heterologous protein production.
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Metabolic engineering of sesquiterpene metabolism in yeast
These yeast now provide a convenient format for investigating catalytic coupling between terpene synthases and hydroxylases, as well as a platform for the industrial production of high value, single‐entity and stereochemically unique terpenes.
Engineering isoflavone metabolism with an artificial bifunctional enzyme
Through a combined molecular modeling, in vitro protein engineering and in planta metabolic engineering approach, it was possible to increase the potential for accumulation of isoflavonoid compounds in non-legume plants.