Heterologous production of secondary metabolites as pharmaceuticals in Saccharomyces cerevisiae

@article{Huang2008HeterologousPO,
  title={Heterologous production of secondary metabolites as pharmaceuticals in Saccharomyces cerevisiae},
  author={Beibei Huang and Jing Guo and Bo Yi and Xiaojing Yu and Lianna Sun and Wansheng Chen},
  journal={Biotechnology Letters},
  year={2008},
  volume={30},
  pages={1121-1137}
}
Heterologous expression of genes involved in the biosynthesis of various products is of increasing interest in biotechnology and in drug research and development. Microbial cells are most appropriate for this purpose. Availability of more microbial genomic sequences in recent years has greatly facilitated the elucidation of metabolic and regulatory networks and helped gain overproduction of desired metabolites or create novel production of commercially important compounds. Saccharomyces… 
Yeast artificial chromosomes employed for random assembly of biosynthetic pathways and production of diverse compounds in Saccharomyces cerevisiae
TLDR
A novel strategy for production of diverse natural products, comprising the expression of an unprecedented large number of biosynthetic genes in a heterologous host is described, particularly suited for generating high numbers of structurally diverse compounds.
Genetically Modified Baker’s Yeast Saccharomyces cerevisiae in Chemical Synthesis and Biotransformations
TLDR
This review focuses on the progress that has been achieved in the production of fine chemicals, bulk chemicals and fuels by genetic manipulation of the enzymatic activity of yeast, the combining of enzyme pathways from different microorganisms into S. cerevisiae and expressing genes from S. Cerevisiae in other hosts.
Engineering the yeast Saccharomyces cerevisiae for the production of L-(+)-ergothioneine
TLDR
The engineering of the baker’s yeast Saccharomyces cerevisiae to produce ergothioneine by fermentation in defined media provides a promising process for the biosynthetic production of ERG.
Engineering the Yeast Saccharomyces cerevisiae for the Production of L-(+)-Ergothioneine
TLDR
The engineering of the baker's yeast Saccharomyces cerevisiae to produce ergothioneine by fermentation in defined media provides a promising process for the biosynthetic production of ERG.
From flavors and pharmaceuticals to advanced biofuels: production of isoprenoids in Saccharomyces cerevisiae.
TLDR
The development of isoprenoid applications from flavors and pharmaceuticals to advanced biofuels is summarized and the strategies to design microbial cell factories are reviewed, focusing on Saccharomyces cerevisiae for the production of these compounds.
Engineering microbial cell factories for the production of plant natural products: from design principles to industrial-scale production
TLDR
Several aspects of PNP production in microbial cell factories are reviewed, including important design principles and recent progress in pathway mining and metabolic engineering, and implemented cases of industrial-scale production of PNPs in microbialcell factories are highlighted.
De novo production of benzyl glucosinolate in Escherichia coli.
Secondary Metabolites from Saccharomyces cerevisiae Species with Anticancer Potential
TLDR
This study shows the significance of bioactive compounds produced by S. cerevisiae species with their possible activity and value in chemotherapeutic drugs pipeline and the isolation and alteration of these natural secondary metabolites would promote the development of chemotherAPEutic drugs.
Heterologous expression and characterization of bacterial 2-C-methyl-d-erythritol-4-phosphate pathway in Saccharomyces cerevisiae
TLDR
This work reports the engineering of Escherichia coli genes encoding the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway into the genome of Saccharomyces cerevisiae and the characterization of intermediate metabolites synthesized by the MEP pathway in yeast and shows that S. Cerevisiae has the capability to functionally express at least some bacterial iron–sulfur cluster proteins in its cytosol.
...
...

References

SHOWING 1-10 OF 84 REFERENCES
Biosynthesis of isoprenoids, polyunsaturated fatty acids and flavonoids in Saccharomyces cerevisiae
TLDR
This review will describe some of the efforts on using S. cerevisiae as a cell factory for the biosynthesis of high-value natural products that belong to the families of isoprenoids, flavonoids and long chain polyunsaturated fatty acids.
Metabolic pathway engineering for complex polyketide biosynthesis in Saccharomyces cerevisiae.
TLDR
This work describes the introduction into S. cerevisiae of pathways for the production of methylmalonyl-coenzyme A (CoA), a precursor for complex polyketides, by both propionyl-CoA-dependent and propiony- CoA-independent routes, and demonstrates that the methylmalonies produced in the engineered yeast strains is used in vivo for theproduction of a polyketide product, a triketide lactone.
Metabolic engineering: techniques for analysis of targets for genetic manipulations.
  • J. Nielsen
  • Biology, Engineering
    Biotechnology and bioengineering
  • 1998
TLDR
Despite the prospect of obtaining major improvement through metabolic engineering, this approach is, however, not expected to completely replace the classical approach to strain improvement-random mutagenesis followed by screening.
Genetic engineering of taxol biosynthetic genes in Saccharomyces cerevisiae.
TLDR
Genetic analysis showed that yeast isoprenoid precursors could be utilized in the reconstituted pathway because products accumulated from the first two engineered pathway steps (leading to the committed intermediate taxadiene); however, a pathway restriction was encountered at the first cytochrome P450 hydroxylation step.
Monoterpenoid biosynthesis in Saccharomyces cerevisiae.
TLDR
The potential use of Saccharomyces cerevisiae as an alternative engineering tool is investigated and it is shown that geraniol synthase expression affects the general ergosterol pathway, but in a manner dependent on the genetic background of the strain.
Microbial isoprenoid production: an example of green chemistry through metabolic engineering.
TLDR
In this chapter, the benefits of using metabolic engineering approaches for the development of green chemistry are illustrated by the recent advances in microbial production of isoprenoids, a diverse and important group of natural compounds with numerous existing and potential commercial applications.
Non-conventional yeasts as hosts for heterologous protein production.
TLDR
The current status of Kluyveromyces lactis, Yarrowia lipolytica, Hansenula polymorpha and Pichia pastoris (the best-known alternative yeast systems) is reviewed and the advantages and limitations of these systems are discussed in relation to S. cerevisiae.
Metabolic engineering of sesquiterpene metabolism in yeast
TLDR
These yeast now provide a convenient format for investigating catalytic coupling between terpene synthases and hydroxylases, as well as a platform for the industrial production of high value, single‐entity and stereochemically unique terpenes.
Engineering isoflavone metabolism with an artificial bifunctional enzyme
TLDR
Through a combined molecular modeling, in vitro protein engineering and in planta metabolic engineering approach, it was possible to increase the potential for accumulation of isoflavonoid compounds in non-legume plants.
...
...