Heterologous pathway assembly reveals molecular steps of fungal terreic acid biosynthesis

@article{Kong2018HeterologousPA,
  title={Heterologous pathway assembly reveals molecular steps of fungal terreic acid biosynthesis},
  author={Chuixing Kong and Hezhou Huang and Ying Xue and Yiqi Liu and Qiangqiang Peng and Qi Liu and Qin Xu and Qiaoyun Zhu and Ying Yin and Xiangshan Zhou and Yuanxing Zhang and Menghao Cai},
  journal={Scientific Reports},
  year={2018},
  volume={8}
}
Terreic acid is a potential anticancer drug as it inhibits Bruton’s tyrosine kinase; however, its biosynthetic molecular steps remain unclear. In this work, the individual reactions of terreic acid biosynthesis were determined by stepwise pathway assembly in a heterologous host, Pichia pastoris, on the basis of previous knockout studies in a native host, Aspergillus terreus. Polyketide synthase AtX was found to catalyze the formation of partially reduced polyketide 6-methylsalicylic acid… 
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