Heterologous expression of taro cystatin protects transgenic tomato against Meloidogyne incognita infection by means of interfering sex determination and suppressing gall formation

@article{Chan2009HeterologousEO,
  title={Heterologous expression of taro cystatin protects transgenic tomato against Meloidogyne incognita infection by means of interfering sex determination and suppressing gall formation},
  author={Yuan-li Chan and Ai-Hwa Yang and Jen-tzu Chen and Kai-Wun Yeh and Ming-Tsair Chan},
  journal={Plant Cell Reports},
  year={2009},
  volume={29},
  pages={231-238}
}
Plant-parasitic nematodes are a major pest of many plant species and cause global economic loss. A phytocystatin gene, Colocasia esculenta cysteine proteinase inhibitor (CeCPI), isolated from a local taro Kaosiang No. 1, and driven by a CaMV35S promoter was delivered into CLN2468D, a heat-tolerant cultivar of tomato (Solanum lycopersicum). When infected with Meloidogyneincognita, one of root-knot nematode (RKN) species, transgenic T1 lines overexpressing CeCPI suppressed gall formation as… 
Pyramiding taro cystatin and fungal chitinase genes driven by a synthetic promoter enhances resistance in tomato to root-knot nematode Meloidogyne incognita.
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A dual gene overexpression system was used to transform tomato in order to provide protection from all growth stages of RKN, demonstrating that transgenic plants had inhibitory effects on RKN that were superior to plants transformed with a single gene.
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Molecular investigation of proteinase inhibitor (PI) gene in tomato plants induced by Meloidogyne species
  • R. Bozbuğa
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  • 2021
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Transplastomic Nicotiana benthamiana plants expressing multiple defence genes encoding protease inhibitors and chitinase display broad-spectrum resistance against insects, pathogens and abiotic stresses.
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SpCYS, a cystatin gene from wild potato (Solanum pinnatisectum), is involved in the resistance against Spodoptera litura
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Bioinformatic analysis revealed that SpCYS encoded protein possessed conserved domains of the cystatin family, and data suggest that Sp CYS is a significant asset for the defense mechanism of potato against S. litura.
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