Heterologous expression of nonribosomal peptide synthetases in B. subtilis: construction of a bi-functional B subtilis/E coli shuttle vector system.

@article{Doekel2002HeterologousEO,
  title={Heterologous expression of nonribosomal peptide synthetases in B. subtilis: construction of a bi-functional B subtilis/E coli shuttle vector system.},
  author={Sascha Doekel and Katrin Eppelmann and Mohamed A. Marahiel},
  journal={FEMS microbiology letters},
  year={2002},
  volume={216 2},
  pages={
          185-91
        }
}
A major obstacle in investigating the biosynthesis of pharmacologically important peptide antibiotics is the heterologous expression of the giant biosynthetic genes. Recently, the genetically engineered strain Bacillus subtilis KE30 has been reported as an excellent surrogate host for the heterologous expression of an entire nonribosomal peptide synthetase (NRPS) gene cluster. In this study, we expand the applicability of this strain, by the development of four Escherichia coli/B. subtilis… CONTINUE READING

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