Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus

  title={Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus},
  author={Saul Nitsche Rocha and Jos{\'e} Abrah{\~a}o-Neto and Mar{\'i}a Esperanza Cerd{\'a}n and Mar{\'i}a Isabel Gonz{\'a}lez-Siso and Andreas K. Gombert},
  booktitle={Microbial cell factories},
BACKGROUND In spite of its advantageous physiological properties for bioprocess applications, the use of the yeast Kluyveromyces marxianus as a host for heterologous protein production has been very limited, in constrast to its close relative Kluyveromyces lactis. In the present work, the model protein glucose oxidase (GOX) from Aspergillus niger was cloned into K. marxianus CBS 6556 and into K. lactis CBS 2359 using three different expression systems. We aimed at verifying how each expression… CONTINUE READING


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