Heterologous expression of a thermophilic esterase in Kluyveromyces yeasts

Abstract

In the present work, a thermophilic esterase from Thermus thermophilus HB27 was cloned into Kluyveromyces marxianus and into Kluyveromyces lactis using two different expression systems, yielding four recombinant strains. K. lactis showed the highest esterase expression levels (294 units per gram dry cell weight, with 65% of cell-bound enzyme) using an… (More)
DOI: 10.1007/s00253-010-2869-8

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