Heterologous expression of a putative K+/H+ antiporter of S. coelicolor A3(2) enhances K+, acidic-pH shock tolerances, and geldanamycin secretion.

@article{Song2013HeterologousEO,
  title={Heterologous expression of a putative K+/H+ antiporter of S. coelicolor A3(2) enhances K+, acidic-pH shock tolerances, and geldanamycin secretion.},
  author={Jae Yang Song and Young Bin Seo and Soon-Kwang Hong and Yong Keun Chang},
  journal={Journal of microbiology and biotechnology},
  year={2013},
  volume={23 2},
  pages={
          149-55
        }
}
Heterologous expression of a putative K+/H+ antiporter of Streptomyces coelicolor A3(2) (designated as sha4) in E. coli and Streptomyces hygroscopicus JCM4427 showed enhanced tolerance to K+ stress, acidic-pH shock, and/or geldanamycin production under K+ stress. In a series of K+ extrusion experiments with sha4-carrying E. coli deficient in the K+/H+ antiporter, a restoration of impaired K+ extrusion activity was observed. Based on this, it was concluded that sha4 was a true K+/H+ antiporter… 
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Mechanisms of response to pH shock in microbial fermentation
TLDR
P pH shock, a novel environmental factor, as a tool for the improvement of fermentation production is highlighted and improvement of the cellular tolerance of genetically engineered bacteria can also be a new field of research in the future to enhance fermentation production.

References

SHOWING 1-10 OF 44 REFERENCES
Roles of putative sodium-hydrogen antiporter (SHA) genes in S. coelicolor A3(2) culture with pH variation.
TLDR
The sha8 gene could confer on the host cell the ability to recover pH to the neutral level after pH variation like a pH drop, and was closely associated with this pH recovery caused by the action ofSha8, whereas actinorhodin production was not due to such pH variation patterns alone.
Expression of the Na+/H+ antiporter gene (g1-nhaC) of alkaliphilic Bacillus sp. G1 in Escherichia coli.
TLDR
The results suggested that the g1-NhaC antiporter from Bacillus sp.
Overexpression of a Na+/H+ antiporter confers salt tolerance on a freshwater cyanobacterium, making it capable of growth in sea water
TLDR
A key role of sodium ion exclusion by the Na+/H+ antiporter for the salt tolerance of photosynhetic organisms is demonstrated.
Function of a Principal Na+/H+ Antiporter, ShaA, Is Required for Initiation of Sporulation in Bacillus subtilis
TLDR
The results suggest that ShaA plays a significant role at an early stage of sporulation and not only during vegetative growth, and raise the possibility that fine control of cytoplasmic ion levels, including control of the internal Na(+) concentration, may be important for the progression of the sporulation process.
Physiological role of nhaB, a specific Na+/H+ antiporter in Escherichia coli.
Characterization of a Na+/H+ antiporter gene of Escherichia coli.
TLDR
A mutant of Escherichia coli with increased Na+/H+ antiport activity was isolated and found by other workers to harbor two mutations, which has now been separated and mapped and characterized cells hearing plasmids containing fragments of a 15-kilobase-pair segment of DNA between carA and dnaJ.
Relationship between the Na+/H+ antiporter and Na+/substrate symport in Bacillus alcalophilus.
TLDR
Monensin, an ionophore that catalyzes Na+/H+ exchange, does not restore respiration-driven Na-substrate symport in KM23 vesicles, and the results suggest that there may be a direct relationship between the antiporter and various Na-/ substrate symporters.
The chromosomal tetracycline resistance locus of Bacillus subtilis encodes a Na+/H+ antiporter that is physiologically important at elevated pH.
Overexpression of wheat Na+/H+ antiporter TNHX1 and H+-pyrophosphatase TVP1 improve salt- and drought-stress tolerance in Arabidopsis thaliana plants.
TLDR
Overexpression of the isolated genes from wheat in Arabidopsis thaliana plants is worthwhile to elucidate the contribution of these proteins to the tolerance mechanism to salt and drought.
Evidence for multiple K+ export systems in Escherichia coli
TLDR
The mutational defect that leads to spontaneous efflux in this strain does not abolish the site(s) responsible for the action of NEM, so this species must have at least one other system, besides those encoded by kefB and kefC, capable of mediating a high rate of K+ efflux.
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