Heterologous expression of L. major proteins in S. cerevisiae: a test of solubility, purity, and gene recoding

Abstract

High level expression of many eukaryotic proteins for structural analysis is likely to require a eukaryotic host since many proteins are either insoluble or lack essential post-translational modifications when expressed in E. coli. The well-studied eukaryote Saccharomyces cerevisiae possesses several attributes of a good expression host: it is simple and… (More)
DOI: 10.1007/s10969-009-9068-9

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