Heterologous expression and optimized production of an Aspergillus aculeatus endo-1,4-beta-mannanase in Yarrowia lipolytica.

@article{Roth2009HeterologousEA,
  title={Heterologous expression and optimized production of an Aspergillus aculeatus endo-1,4-beta-mannanase in Yarrowia lipolytica.},
  author={R Roth and Venessa Moodley and Petrus J van Zyl},
  journal={Molecular biotechnology},
  year={2009},
  volume={43 2},
  pages={112-20}
}
The Aspergillus aculeatus MRC11624 man1 gene, encoding an endo-beta-1,4-mannanase, was cloned and expressed in the promising heterologous enzyme producer, the ascomycetous yeast Yarrowia lipolytica. Both single- and multi-copy transformants were constructed, and the secretion of the enzyme was evaluated as an in-frame fusion with the LIP2 secretion signal, as well as with its natural secretion signal. In shake-flask analysis, the highest volumetric enzyme activity (13,073 nkat/ml) and specific… CONTINUE READING
12 Citations
30 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 12 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 30 references

Molecular cloning: A laboratory manual (3rd ed.)

  • J. Sambrook, E. F. Fitsch, T. Maniatis
  • Cold Spring Harbor,
  • 1989
Highly Influential
12 Excerpts

Expression of the Aspergillus aculeatus endo-b-1, 4-mannanse encoding gene (man1) in Saccharomyces cerevisiae and characterization of the recombinant protein

  • M. E. Setati, P. Ademark, W. H. van Zyl, B. Hahn-Hägerdal, H. Stålbrand
  • Protein Expression and Purification,
  • 2001
Highly Influential
9 Excerpts

Production of the Aspergillus aculeatus endo - 1 , 4 - b - mannanase in A . niger

  • P. J. Van Zyl, V. Moodley, S. H. Rose, R. L. Roth, W. H. van Zyl
  • Journal of Industrial Microbiology…
  • 2009

Similar Papers

Loading similar papers…