Heterologous Expression of Alteromonas macleodii and Thiocapsa roseopersicina [NiFe] Hydrogenases in Synechococcus elongatus

@article{Weyman2011HeterologousEO,
  title={Heterologous Expression of Alteromonas macleodii and Thiocapsa roseopersicina [NiFe] Hydrogenases in Synechococcus elongatus},
  author={Philip D Weyman and Walter A Vargas and Yingkai Tong and Jianping Yu and Pin-Ching Maness and Hamilton O. Smith and Qing Xu},
  journal={PLoS ONE},
  year={2011},
  volume={6},
  pages={71 - 91}
}
  • Philip D Weyman, Walter A Vargas, +4 authors Qing Xu
  • Published in PloS one 2011
  • Medicine, Biology
  • PLoS ONE
  • Oxygen-tolerant [NiFe] hydrogenases may be used in future photobiological hydrogen production systems once the enzymes can be heterologously expressed in host organisms of interest. To achieve heterologous expression of [NiFe] hydrogenases in cyanobacteria, the two hydrogenase structural genes from Alteromonas macleodii Deep ecotype (AltDE), hynS and hynL, along with the surrounding genes in the gene operon of HynSL were cloned in a vector with an IPTG-inducible promoter and introduced into… CONTINUE READING

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    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 40 REFERENCES

    Heterologous Expression of Alteromonas macleodii and Thiocapsa roseopersicina [ NiFe ] Hydrogenases in E . coli

    • PD Weyman, WA Vargas, RY Chuang, Y Chang, HO Smith
    • Microbiology
    • 2011

    Discovery of a [NiFe]-hydrogenase in metagenomic Sargasso Sea DNA: Cloning and Functional Analysis in Thiocapsa roseopersicina

    • G Maroti, Y Tong, S Yooseph, H Baden-Tillson, HO Smith
    • Appl Environ Microbiol
    • 2009
    VIEW 2 EXCERPTS