Heterologous Expression and Characterization of an Acidic GH11 Family Xylanase from Hypocrea orientalis.

Abstract

A gene encoding glycoside hydrolase family 11 xylanase (HoXyn11B) from Hypocrea orientalis EU7-22 was expressed in Pichia pastoris with a high activity (413 IU/ml). HoXyn11B was partly N-glycosylated and appeared two protein bands (19-29 kDa) on SDS-PAGE. The recombinant enzyme exhibited optimal activity at pH 4.5 and 55 °C, and retained more than 90% of… (More)
DOI: 10.1007/s12010-017-2532-2

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