Heterogeneous response of cytoplasmic free Ca2+ in proximal convoluted and straight tubule cells in primary culture.

@article{Suzuki1989HeterogeneousRO,
  title={Heterogeneous response of cytoplasmic free Ca2+ in proximal convoluted and straight tubule cells in primary culture.},
  author={Masahiro Suzuki and Yoshindo Kawaguchi and Satoshi Kurihara and Toshihiko Miyahara},
  journal={The American journal of physiology},
  year={1989},
  volume={257 5 Pt 2},
  pages={F724-31}
}
Cytoplasmic free calcium [( Ca2+]c) plays an important role in cellular signal transduction in response to hormones. The development of microfluorometric digital image analysis makes it possible to obtain an image of a calcium-indicator dye, fura-2, in a single living cell. Subconfluent primary cultured cells from isolated proximal convoluted and straight tubules were analyzed for [Ca2+]c. The basal level of [Ca2+]c was 92.0 +/- 0.82 nM in convoluted and 75.0 +/- 4.7 nM in straight tubule cells… CONTINUE READING