Heterogeneity of young and aged murine hematopoietic stem cells revealed by quantitative clonal analysis using cellular barcoding.

@article{Verovskaya2013HeterogeneityOY,
  title={Heterogeneity of young and aged murine hematopoietic stem cells revealed by quantitative clonal analysis using cellular barcoding.},
  author={Evgenia Verovskaya and Mathilde J. C. Broekhuis and Erik W. Zwart and Martha Ritsema and Ronald P. van Os and Gerald de Haan and Leonid V. Bystrykh},
  journal={Blood},
  year={2013},
  volume={122 4},
  pages={523-32}
}
The number of hematopoietic stem cells (HSCs) that contributes to blood formation and the dynamics of their clonal contribution is a matter of ongoing discussion. Here, we use cellular barcoding combined with multiplex high-throughput sequencing to provide a quantitative and sensitive analysis of clonal behavior of hundreds of young and old HSCs. The majority of transplanted clones steadily contributes to hematopoiesis in the long-term, although clonal output in granulocytes, T cells, and B… CONTINUE READING
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