Heterogeneity of unfractionated heparins studied in connection with species, source, and production processes.

@article{Bianchini1997HeterogeneityOU,
  title={Heterogeneity of unfractionated heparins studied in connection with species, source, and production processes.},
  author={P. Bianchini and L. Liverani and G. Mascellani and B. Parma},
  journal={Seminars in thrombosis and hemostasis},
  year={1997},
  volume={23 1},
  pages={
          3-10
        }
}
The heterogeneity of unfractionated heparins (Hep) can be correlated to the species and organs of origin and to the process of production. Heparins, extracted by different, validated processes from different organs and/or tissues (mucosa, thymus, pancreas, placenta, lung, intestine) or mammals (pig, beef, sheep, man) and other vertebrates (chicken), have been examined by HPLC analysis of heparinase digests. By analysis of disaccharides many observations have been made. Porcine mucosa heparin… Expand
Structural peculiarity and antithrombin binding region profile of mucosal bovine and porcine heparins.
TLDR
A combined study by 2D (heteronuclear single quantum coherence, HSQC) NMR and ion-pair reversed-phase high performance liquid chromatography coupled with electrospray ionization mass spectrometry (ESI-MS) on the heparinases confirmed the previously reported lower degree of 6-O-sulfation and showed lower 3-Sulfated glucosamine content in BMH. Expand
By-Products of Heparin Production Provide a Diverse Source of Heparin-like and Heparan Sulfate Glycosaminoglycans
TLDR
Collective analyses indicate that the by-products of standard heparin production represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products. Expand
Quantitative PCR and Disaccharide Profiling to Characterize the Animal Origin of Low-Molecular-Weight Heparins
TLDR
A quantitative polymerase chain reaction method has been developed to ensure the purity of the porcine origin of crude heparin, with a DNA detection limit as low as 1 ppm for bovine, or 10 ppm for ovine contaminants. Expand
Turkey intestine as a commercial source of heparin? Comparative structural studies of intestinal avian and mammalian glycosaminoglycans.
TLDR
Structural characterization studies clearly demonstrate that heparan sulfate is the major GAG in the turkey intestine, and this observation is rationalized based on differences in the mammalian and avian coagulation and immune systems. Expand
Structural and haemostatic features of pharmaceutical heparins from different animal sources: challenges to define thresholds separating distinct drugs
TLDR
It is reasonably indicated that pharmaceutical preparations of heparin from different animal sources constitute distinct drugs, thus requiring specific regulatory rules and therapeutic evaluations. Expand
Characterisation of Ag1, the major species-specific contaminant of bovine crude heparin, and its identification as an aprotinin/heparin complex.
TLDR
The stability of Ag1 towards the different treatments performed during heparin extraction process allows this protein to remain in sufficient amounts in crude heparins and makes it an ideal target for the immunochemical control of the absence of bovine material in crude Heparin. Expand
Variability of heparins and heterogeneity of low molecular weight heparins.
TLDR
The reported examples prove that the variability among samples of LMWHs manufactured by the same process is quite limited, and most of the variability is derived from the parent UFH. Expand
Biochemical and functional characterization of glycosaminoglycans released from degranulating rat peritoneal mast cells: Insights into the physiological role of endogenous heparin.
TLDR
It is shown that rat peritoneal mast cells contain a mixture of GAGs that possess anticoagulant and anti-inflammatory properties. Expand
Bovine Mucosal Heparins Are Comparable to Porcine Mucosal Heparin at USP Potency Adjusted Levels
TLDR
BMH, when used at comparable anti-Xa unit levels, is comparable to PMH, however, it requires proportionally higher amount of protamine due to the increased mass for adjusting to higher potency. Expand
Heparin mimetics with anticoagulant activity
TLDR
This review encompasses the development of heparin mimetics of various structural classes, including synthetic polymers and non‐carbohydrate small molecules as well as sulfated oligo‐ and polysaccharides, and fondaparinux derivatives and conjugates, with a focus on developments in the past 10 years. Expand
...
1
2
3
4
5
...