Heterogeneity of the glycans O-glycosidically linked to the hinge region of secretory immunoglobulins from human milk.

@article{PierceCrtel1981HeterogeneityOT,
  title={Heterogeneity of the glycans O-glycosidically linked to the hinge region of secretory immunoglobulins from human milk.},
  author={A Pierce-Cr{\'e}tel and Merc{\`e} Pamblanco and G{\'e}rard Strecker and Jean Montreuil and Genevi{\'e}ve Spik},
  journal={European journal of biochemistry},
  year={1981},
  volume={114 1},
  pages={169-78}
}
Pure secretory immunoglobulin A was isolated from human milk by fractionation in gradients of pH and (NH4)2SO4 concentration followed by gel filtration. The hinge region containing all the O-glycosidically linked oligosaccharides was isolated en bloc after trypsin and pepsin hydrolysis and separated by gel filtration. The mixture of O-glycosidically linked oligosaccharides contained N-acetylneuraminic acid (NeuAc), fucose (Fuc), galactose (Gal), N-acetylglucosamine (GlcNAc) and N… CONTINUE READING

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