Heterogeneity of the complex N-linked oligosaccharides at specific glycosylation sites of two secreted carrot glycoproteins.

@article{Sturm1991HeterogeneityOT,
  title={Heterogeneity of the complex N-linked oligosaccharides at specific glycosylation sites of two secreted carrot glycoproteins.},
  author={Arnd Sturm},
  journal={European journal of biochemistry},
  year={1991},
  volume={199 1},
  pages={169-79}
}
  • Arnd Sturm
  • Published 1991 in European journal of biochemistry
The N-linked glycans from the 52/54-kDa medium protein and cell wall beta-fructosidase, two glycoproteins secreted by carrot suspension culture cells, were characterized. Carrot cells were labelled with [3H]glucosamine or [3H]fucose. The 52/54-kDa medium protein was isolated from the culture medium and beta-fructosidase from cell walls. The purified proteins were digested with trypsin and glycopeptides were isolated and sequenced. Glycans obtained from individual glycopeptides were separated by… CONTINUE READING