Heterogeneity of phosphatidic acid levels and distribution at the plasma membrane in living cells as visualized by a Föster resonance energy transfer (FRET) biosensor.

@article{Nishioka2010HeterogeneityOP,
  title={Heterogeneity of phosphatidic acid levels and distribution at the plasma membrane in living cells as visualized by a F{\"o}ster resonance energy transfer (FRET) biosensor.},
  author={Teruko Nishioka and Michael A. Frohman and Michiyuki Matsuda and Etsuko Kiyokawa},
  journal={The Journal of biological chemistry},
  year={2010},
  volume={285 46},
  pages={35979-87}
}
Phosphatidic acid (PA) is one of the major phospholipids in the plasma membrane. Although it has been reported that PA plays key roles in cell survival and morphology, it remains unknown when and where PA is produced in the living cell. Based on the principle of Förster resonance energy transfer (FRET), we generated PA biosensor, and named Pii (phosphatidic acid indicator). In these biosensors, the lipid-binding domain of DOCK2 is sandwiched with the cyan fluorescent protein and yellow… CONTINUE READING

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1 Excerpt

FRET Probe for Phosphatidic Acid 35986 JOURNAL OF BIOLOGICAL CHEMISTRY VOLUME

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