Heterogeneity and contact-dependent regulation of hormone secretion by individual B cells.

A reverse hemolytic plaque assay was developed to visualize insulin release from individual adult pancreatic B cells. Cells obtained by mechanical dispersion of isolated rat islets of Langerhans were mixed with protein A-coated sheep red blood cells and incubated in the presence of an anti-insulin serum, under conditions known to affect insulin release. The… CONTINUE READING