Heteroduplex strand-specificity in restriction-stimulated recombination by the RecE pathway of Escherichia coli.

@article{Silberstein1993HeteroduplexSI,
  title={Heteroduplex strand-specificity in restriction-stimulated recombination by the RecE pathway of Escherichia coli.},
  author={Zipora Silberstein and Moshe Shalit and Adrian M Cohen},
  journal={Genetics},
  year={1993},
  volume={133 3},
  pages={439-48}
}
The RecE recombination pathway is active in Escherichia coli recB recC sbcA mutants. To isolate and characterize products and intermediates of RecE-mediated, break-induced, intramolecular recombination, we infected recB recC sbcA mutants, expressing EcoRI endonuclease, with chimeric lambda phages that allow EcoRI-mediated release of cloned linear recombination substrates. Substrates with direct terminal repeats recombined to yield a circular product with one copy of the repeated sequence. Some… CONTINUE READING

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