Heteroduplex polymerase chain reaction is essential for canine receptor rearrangement analysis.

@article{Takanosu2010HeteroduplexPC,
  title={Heteroduplex polymerase chain reaction is essential for canine receptor rearrangement analysis.},
  author={Masamine Takanosu and Tomohiko Tadika and Tetsuya Kobayashi},
  journal={Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc},
  year={2010},
  volume={22 5},
  pages={760-3}
}
Polymerase chain reaction (PCR) for antigen receptor rearrangement is a sensitive technique for detecting lymphocyte-proliferative disorders, but it tends to produce false-positive results, a phenomenon termed pseudoclonality. Heteroduplex analysis, which is useful to distinguish clonal reactions from pseudoclonal ones in dogs, can be applied to avoid misdiagnosis and determine the reliability of results. In the current study, PCR for antigen receptor rearrangement was used to identify clonal… CONTINUE READING