Heteroduplex cleavage analysis using S1 nuclease.

@article{Howard1999HeteroduplexCA,
  title={Heteroduplex cleavage analysis using S1 nuclease.},
  author={Joelyn Tonkin Howard and John V Ward and Jacqueline N Watson and Kenneth H. Roux},
  journal={BioTechniques},
  year={1999},
  volume={27 1},
  pages={18-9}
}
A variety of methods have been developed to screen for genetic mutations. Most rely on the comparative analysis of polymerase chain reaction (PCR) products. Single-strand conformational polymorphism (4), the most widely used technique, relies on sequence-dependent conformational electrophoretic mobility differences between wild-type (WT) and mutant strands; whereas, heteroduplex analysis (5,8), the second most popular technique, is based on differential mobility between homoduplexes and… CONTINUE READING
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