Heterodimerization of the yeast homeodomain transcriptional regulators alpha 2 and a1 induces an interfacial helix in alpha 2.

@article{Phillips1994HeterodimerizationOT,
  title={Heterodimerization of the yeast homeodomain transcriptional regulators alpha 2 and a1 induces an interfacial helix in alpha 2.},
  author={Carrie L. Phillips and Martha R. Stark and Alexander Johnson and Frederick W. Dahlquist},
  journal={Biochemistry},
  year={1994},
  volume={33 31},
  pages={9294-302}
}
The homeodomain proteins a1 and alpha 2 act cooperatively to regulate cell type specific genes in yeast. The basis of the cooperativity is a weak interaction between the two proteins which forms heterodimers that bind DNA tightly and specifically. In this paper, we examine the mechanism of heterodimerization. We show that two relatively small fragments of a1 and alpha 2 are capable of heterodimerization and tight DNA binding. The alpha 2 fragment contains the homeodomain followed by the natural… CONTINUE READING