Heterobifunctional PEG Ligands for Bioconjugation Reactions on Iron Oxide Nanoparticles

Abstract

Ever since iron oxide nanoparticles have been recognized as promising scaffolds for biomedical applications, their surface functionalization has become even more important. We report the synthesis of a novel polyethylene glycol-based ligand that combines multiple advantageous properties for these applications. The ligand is covalently bound to the surface via a siloxane group, while its polyethylene glycol backbone significantly improves the colloidal stability of the particle in complex environments. End-capping the molecule with a carboxylic acid introduces a variety of coupling chemistry possibilities. In this study an antibody targeting plasminogen activator inhibitor-1 was coupled to the surface and its presence and binding activity was assessed by enzyme-linked immunosorbent assay and surface plasmon resonance experiments. The results indicate that the ligand has high potential towards biomedical applications where colloidal stability and advanced functionality is crucial.

DOI: 10.1371/journal.pone.0109475

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@inproceedings{Bloemen2014HeterobifunctionalPL, title={Heterobifunctional PEG Ligands for Bioconjugation Reactions on Iron Oxide Nanoparticles}, author={Maarten Bloemen and Thomas Van Stappen and Pieter Willot and Jeroen Lammertyn and Guy Koeckelberghs and Nick Geukens and Ann Gils and Thierry Verbiest and Maxim Antopolsky}, booktitle={PloS one}, year={2014} }