Herpes simplex virus immediate early infected-cell polypeptide 4 binds to DNA and promotes transcription.

@article{Beard1986HerpesSV,
  title={Herpes simplex virus immediate early infected-cell polypeptide 4 binds to DNA and promotes transcription.},
  author={Peter Beard and Steven Faber and Kent W Wilcox and Lewis I. Pizer},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1986},
  volume={83 11},
  pages={4016-20}
}
In herpes simplex virus (HSV)-infected cells, there is a sequential expression of viral genes. In vivo experiments have implicated the Mr 175,000 immediate early protein ICP4 (infected-cell polypeptide 4) in the regulation of viral RNA synthesis, but the mechanism whereby ICP4 regulates transcription of viral genes is at present unknown. In this report we describe experiments with an in vitro transcription system and a purified preparation of ICP4 (estimated 5% of total protein). Using DNA from… CONTINUE READING