Herpes simplex virus as a transneuronal tracer

  title={Herpes simplex virus as a transneuronal tracer},
  author={Robert B. Norgren and MichaelN . Lehman},
  journal={Neuroscience \& Biobehavioral Reviews},

Viral Tracers for the Analysis of Neural Circuits

This chapter discusses how the pseudorabies virus (PRV) strain Bartha, an attenuated form of a pig alphaherpesvirus, is an excellent retrograde transneuronal tracer for labeling neural networks in central nervous system networks involving chains of two or more functionally connected neurons.

Restrictions that control herpes simplex virus type 1 infection in mouse brain ex vivo.

It is concluded that tissue resistance to infection is associated with a block at or prior to the immediate-early mRNA synthesis, which indicates that HSV-1 genes are not expressed in regions that do not express a viral reporter gene.

Transneuronal retrograde transport of attenuated pseudorabies viruses within central visual pathways

The PRV strains used produce a local infection that progresses primarily in the retrograde direction in the central visual pathways, providing a means of examining detailed circuitry within the visual pathway.

Herpes simplex type 1 infects and establishes latency in the brain and trigeminal ganglia during primary infection of the lip in cotton rats and mice

Using PCR and RT-PCR techniques, HSV-1 thymidine kinase is demonstrated in the absence of infectivity in the brains of both species 30–40 days after primary infection.

Dual Transneuronal Tracing in the Rat Entorhinal-Hippocampal Circuit by Intracerebral Injection of Recombinant Rabies Virus Vectors

It is established that single neurons in CA3 are connected to the entorhinal cortex bilaterally with only one synaptic relay, and dual transneuronal tracing potentially provides a powerful and sensitive method to study issues such as the amount of convergence and divergence within and between circuits in the central nervous system.

Use of Adeno‐Associated and Herpes Simplex Viral Vectors for In Vivo Neuronal Expression in Mice

Methods for storing, delivering, and verifying expression of adeno‐associated and herpes simplex viruses in the adult mouse brain and important considerations for experiments using in vivo expression of these viral vectors, including serotype and promoter selection, are described.

Transsynaptic tracing by in situ complementation of a deletion mutant neurotropic virus

Two new tools for tracing neural connections are developed that should allow a significantly more precise understanding of the organization of nervous systems than has previously been possible.



The roles of herpes simplex virus in neuroscience.

The goal of this paper is to review the different roles for HSV in the broad field of neuroscience and the construction and development of HSV-based vectors for gene therapy and the use ofHSV as a neuronal tracer.

Direction of transneuronal transport of herpes simplex virus 1 in the primate motor system is strain-dependent.

The observations suggest that strain differences have an important impact on the direction of transneuronal transport of HSV-1 and virus strain differences, and it should be possible to examine the organization of cerebellar and basal ganglia loops with cerebral cortex by exploiting transneurononal transport.

Neurobiology of human herpesvirus infections.

This review examines current understanding of HSV-1 infections of both the peripheral and CNS from a neurobiological perspective and concludes that herpes encephalitis represents an aberrant interaction of the virus and host that is at present poorly understood.

Herpes simplex virus infection of motor neurons: hypoglossal model

Findings indicate that HSV can penetrate the neuromuscular junction, travel in a pure motor nerve, and produce a focal encephalitis in the corresponding central nervous system motor nucleus.

The transneuronal spread phenotype of herpes simplex virus type 1 infection of the mouse hind footpad

It is postulate that hindlimb paralysis may have resulted from intense involvement of the posterior column (gracile fasciculus) in the thoracolumbar spinal cord, a region known to contain the corticospinal tract in rodents.

A quantitative assay of retrograde transported HSV in the trigeminal ganglion.

The relationship between the dose of Herpes simplex virus type 1 (HSV) inoculated in the cornea and the amount of actively replicating virus recovered from mouse trigeminal ganglion cells 5 d after