Heritable gene targeting in zebrafish using customized TALENs

  title={Heritable gene targeting in zebrafish using customized TALENs},
  author={Peng Huang and An Xiao and Mingguo Zhou and Zuoyan Zhu and Shuo Lin and Bo Zhang},
  journal={Nature Biotechnology},
699 To the Editor: Studies of targeted gene modifications are of great interest in basic research as well as for clinical and agricultural applications1. In the February issue of Nature Biotechnology, two articles reported genomic modifications using transcription activator-like (TAL) effectors2,3. Using fusion proteins, each comprising a TAL effector DNA binding domain and a FokI cleavage domain, Miller et al.2 reported that TAL effector nucleases (TALENs) successfully disrupted target genes… 

In vivo Genome Editing Using High Efficiency TALENs

Improvements in artificial transcription activator-like effector nucleases (TALENs) provide a powerful new approach for targeted zebrafish genome editing and functional genomic applications and offer the potential to model genetic variation as well as to generate targeted conditional alleles.

In vivo genome editing using a high-efficiency TALEN system

Improvements in artificial transcription activator-like effector nucleases (TALENs) provide a powerful new approach for targeted zebrafish genome editing and functional genomic applications and this enhanced TALEN toolkit demonstrates a high efficiency in inducing locus-specific DNA breaks in somatic and germline tissues.

High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos

The results demonstrate that targeted gene disruption by TALENs provides a method comparable to antisense morpholinos in analyzing gene function in Xenopus F0 embryos, but also applies beyond embryogenesis to any life stage.

A library of TAL effector nucleases spanning the human genome

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TALENs: customizable molecular DNA scissors for genome engineering of plants.

Efficient Targeted Mutagenesis in Medaka Using Custom-Designed Transcription Activator-Like Effector Nucleases

The results suggest that TALENs enable us to rapidly and efficiently establish knockout medaka strains and will expand the potential of medaka as a model system for genetics and genomics.

Endonucleases: new tools to edit the mouse genome.

Efficient Knockout of Transplanted Green Fluorescent Protein Gene in Medaka Using TALENs

It is shown that TALENs can directly knock out a green fluorescent protein (GFP) transgene in medaka by affecting translation and synthesis of the GFP.

An optimized TALEN application for mutagenesis and screening in Drosophila melanogaster

A simple methodology to quantitatively assess bi-allelic TALEN cutting efficiency, as well as approaches that permit accurate measures of somatic and germline mutation rates in Drosophila melanogaster, provide an effective step-wise guide for routine TALen-mediated gene editing in the fly.



Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases

The use of engineered ZFNs to introduce heritable mutations into a genome obviates the need for embryonic stem cell lines and should be applicable to most animal species for which early-stage embryos are easily accessible.

Heritable Targeted Gene Disruption in Zebrafish Using Designed Zinc Finger Nucleases

The frequency and precision of gene-disruption events observed suggest that this approach should be applicable to any loci in zebrafish or in other organisms that allow mRNA delivery into the fertilized egg.

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting

A method and reagents for efficiently assembling TALEN constructs with custom repeat arrays are presented and design guidelines based on naturally occurring TAL effectors and their binding sites are described.

Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes

These studies expand the realm of verified TALEN activity from cultured human cells to an intact eukaryotic organism and suggest that low-cost, highly dependable dTALENs can assume a significant role for gene modifications of value in human and animal health, agriculture and industry.

Regulation of selected genome loci using de novo-engineered transcription activator-like effector (TALE)-type transcription factors

The data demonstrate that the TALE scaffold can be tailored to target user-defined DNA sequences in whole genomes and mediates specific interaction with G nucleotides that thus far could not be targeted specifically by any known RVD type.

Transcriptional Activators of Human Genes with Programmable DNA-Specificity

Synthetic TAL proteins with designed repeat compositions were created using a novel modular cloning strategy termed “Golden TAL Technology” and activated targeted expression of exogenous as well as endogenous genes.

Assembly of Designer TAL Effectors by Golden Gate Cloning

This work presents here a strategy for engineering of TALE proteins with novel DNA binding specificities based on the 17.5 repeat-containing AvrBs3 TALE as a scaffold, and assembled designer TALEs with new target specificities and tested their function in vivo.

A TALE nuclease architecture for efficient genome editing

This study identifies TALE truncation variants that efficiently cleave DNA when linked to the catalytic domain of FokI and uses them to generate discrete edits or small deletions within endogenous human NTF3 and CCR5 genes at efficiencies of up to 25%.

Targeted Genome Editing Across Species Using ZFNs and TALENs

A broadly applicable strategy using zinc finger nucleases and transcription activator-like effector nucleases for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species is established.

Genetic engineering of human ES and iPS cells using TALE nucleases

The data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs).