Heritable gene targeting in zebrafish using customized TALENs

@article{Huang2011HeritableGT,
  title={Heritable gene targeting in zebrafish using customized TALENs},
  author={Peng Huang and An Xiao and Mingguo Zhou and Zuoyan Zhu and Shuo Lin and Bo Zhang},
  journal={Nature Biotechnology},
  year={2011},
  volume={29},
  pages={699-700}
}
699 To the Editor: Studies of targeted gene modifications are of great interest in basic research as well as for clinical and agricultural applications1. In the February issue of Nature Biotechnology, two articles reported genomic modifications using transcription activator-like (TAL) effectors2,3. Using fusion proteins, each comprising a TAL effector DNA binding domain and a FokI cleavage domain, Miller et al.2 reported that TAL effector nucleases (TALENs) successfully disrupted target genes… Expand
In vivo Genome Editing Using High Efficiency TALENs
TLDR
Improvements in artificial transcription activator-like effector nucleases (TALENs) provide a powerful new approach for targeted zebrafish genome editing and functional genomic applications and offer the potential to model genetic variation as well as to generate targeted conditional alleles. Expand
In vivo genome editing using a high-efficiency TALEN system
The zebrafish (Danio rerio) is increasingly being used to study basic vertebrate biology and human disease using a rich array of in vivo genetic and molecular tools. However, the inability to readilyExpand
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TLDR
The results demonstrate that targeted gene disruption by TALENs provides a method comparable to antisense morpholinos in analyzing gene function in Xenopus F0 embryos, but also applies beyond embryogenesis to any life stage. Expand
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TLDR
A genome-scale collection of TALENs for efficient and scalable gene targeting in human cells is presented and single- and double-gene-knockout cells in which NF-κB signaling pathways were disrupted showed unambiguous suppression of signal transduction. Expand
TALENs: customizable molecular DNA scissors for genome engineering of plants.
  • Kunling Chen, C. Gao
  • Biology, Medicine
  • Journal of genetics and genomics = Yi chuan xue bao
  • 2013
TLDR
TALENs are reviewed, the principles and tools for TALEN-mediated gene targeting in plant cells are summarized, and current and potential strategies for use in plant research and crop improvement are summarized. Expand
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TLDR
The results suggest that TALENs enable us to rapidly and efficiently establish knockout medaka strains and will expand the potential of medaka as a model system for genetics and genomics. Expand
An efficient TALEN mutagenesis system in rice.
TLDR
A detailed protocol for constructing TALEN expression vectors, assessing nuclease activities in vivo using rice protoplast-based assays, generating and introducing TalEN DNAs into embryogenic calluses of rice and identifying TALen-generated mutations at targeted genomic sites is described. Expand
Use of TALEs and TALEN Technology for Genetic Improvement of Plants
TLDR
TALEs and TALENs, their discovery, binding specificity, designing, functional domains, delivery and use for genome editing specifically in plants are reviewed. Expand
Efficient Knockout of Transplanted Green Fluorescent Protein Gene in Medaka Using TALENs
TLDR
It is shown that TALENs can directly knock out a green fluorescent protein (GFP) transgene in medaka by affecting translation and synthesis of the GFP. Expand
Endonucleases: new tools to edit the mouse genome.
TLDR
The basic principles of these new strategies in mouse genome manipulation, their inherent advantages, and their potential disadvantages compared to current technologies used to study gene function in mouse models are described. Expand
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The use of engineered ZFNs to introduce heritable mutations into a genome obviates the need for embryonic stem cell lines and should be applicable to most animal species for which early-stage embryos are easily accessible. Expand
Heritable Targeted Gene Disruption in Zebrafish Using Designed Zinc Finger Nucleases
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The frequency and precision of gene-disruption events observed suggest that this approach should be applicable to any loci in zebrafish or in other organisms that allow mRNA delivery into the fertilized egg. Expand
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting
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A method and reagents for efficiently assembling TALEN constructs with custom repeat arrays are presented and design guidelines based on naturally occurring TAL effectors and their binding sites are described. Expand
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These studies expand the realm of verified TALEN activity from cultured human cells to an intact eukaryotic organism and suggest that low-cost, highly dependable dTALENs can assume a significant role for gene modifications of value in human and animal health, agriculture and industry. Expand
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TLDR
The data demonstrate that the TALE scaffold can be tailored to target user-defined DNA sequences in whole genomes and mediates specific interaction with G nucleotides that thus far could not be targeted specifically by any known RVD type. Expand
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TLDR
Synthetic TAL proteins with designed repeat compositions were created using a novel modular cloning strategy termed “Golden TAL Technology” and activated targeted expression of exogenous as well as endogenous genes. Expand
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TLDR
This work presents here a strategy for engineering of TALE proteins with novel DNA binding specificities based on the 17.5 repeat-containing AvrBs3 TALE as a scaffold, and assembled designer TALEs with new target specificities and tested their function in vivo. Expand
A TALE nuclease architecture for efficient genome editing
TLDR
This study identifies TALE truncation variants that efficiently cleave DNA when linked to the catalytic domain of FokI and uses them to generate discrete edits or small deletions within endogenous human NTF3 and CCR5 genes at efficiencies of up to 25%. Expand
Targeted Genome Editing Across Species Using ZFNs and TALENs
TLDR
A broadly applicable strategy using zinc finger nucleases and transcription activator-like effector nucleases for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species is established. Expand
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