Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins

@inproceedings{Cho2013HeritableGK,
  title={Heritable Gene Knockout in Caenorhabditis elegans by Direct Injection of Cas9–sgRNA Ribonucleoproteins},
  author={Seung Woo Cho and Jihyun Lee and Dana Carroll and Jin-Soo Kim and Junho Lee},
  booktitle={Genetics},
  year={2013}
}
We present a novel method of targeted gene disruption that involves direct injection of recombinant Cas9 protein complexed with guide RNA into the gonad of the nematode Caenorhabditis elegans. Biallelic mutants were recovered among the F1 progeny, demonstrating the high efficiency of this method. 

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