Heritable CRISPR/Cas9-mediated targeted integration in Xenopus tropicalis.

  title={Heritable CRISPR/Cas9-mediated targeted integration in Xenopus tropicalis.},
  author={Zhaoying Shi and Fengqin Wang and Yan Cui and Zhongzhen Liu and Xiaogang Guo and Yanqi Zhang and Yi Deng and Hui Zhao and Yonglong Chen},
  journal={FASEB journal : official publication of the Federation of American Societies for Experimental Biology},
  volume={29 12},
Xenopus tropicalis is an emerging vertebrate genetic model. A gene knock-in method has not yet been reported in this species. Here, we report that heritable targeted integration can be achieved in this diploid frog using a concurrent cleavage strategy mediated by the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (CRISPR/Cas9) system. The key point of the strategy is the addition of a Cas9/guide RNA cleavage site in the donor vector, allowing… CONTINUE READING