Protective effect of a polyherbal mixture was evaluated for their hepatoprotective activity against carbon tetrachloride, D-galactosamine intoxication. Biochemical estimations for AST, ALT, ALP, Total bilirubin and Total protein were carried out at the end of treatment schedule for respective models. Three mixtures of equivalent amounts the extracts of Picrorhiza kurrao, Embilica officinalis, Andrographis paniculata, Eclipta alba to constitute total doses T25mg, T50 mg and T100 mg were prepared. Carbon tetrachloride induced hepatotoxicity was used as pilot model to determine effective dose. The pilot study showed significant (p<0.01) hepatoprotective activity at doses of T50 mg and T100 mg of mixture. Whereas T25 mg mixture dose was insignificant. The minimal effective dose of T50 mg of mixture was taken as the test dose for evaluation in D-galactosamine induced hepatotoxicity. In carbon tetrachloride induced hepatotoxicity, T50 mg and T100 mg mixture doses showed significant (p<0.01) restoration of SGOT, SGPT, ALP, Total bilirubin and Total protein levels at the end of treatment schedule. T50 mg mixture dose in D-galactosamine induced hepatotoxicity also showed significant (p<0.01) restoration of AST, ALT, ALP, Total bilirubin and Total protein levels at the end of treatment schedule. The above study suggest that T50 mg and T100 mg of mixture of extracts of above said plants have hepatoprotective against lipid peroxidation of hepatocytes against free radicals generated in carbon tetrachloride intoxication. In T-50 mg treated group the levels of ALT, ALP, TB were restored significantly (p<0.01) to the normal levels indicating the membrane stabilizing capacity of the herbal mixture in D-galactosamine induced hepatotoxicity.