Hepatocytic cells form bile duct-like structures within a three-dimensional collagen gel matrix.

@article{Nishikawa1996HepatocyticCF,
  title={Hepatocytic cells form bile duct-like structures within a three-dimensional collagen gel matrix.},
  author={Yuji Nishikawa and Yoshihiko Tokusashi and Takayuki Kadohama and Hiroyuki Nishimori and Kazushige Ogawa},
  journal={Experimental cell research},
  year={1996},
  volume={223 2},
  pages={357-71}
}
It has been suggested that hepatocytes have the ability to form bile ductal structures during normal development and in various pathological conditions of the liver. In the present study, we attempted to establish an in vitro model of ductal morphogenesis of hepatocytic cells by combining an aggregate culture and a type I collagen gel culture. When spheroidal aggregates of rat or mouse primary hepatocytes were embedded within the collagen gel matrix and then cultured with a medium containing a… CONTINUE READING