Hepatocyte nuclear factor-1α regulates transcription of the guanylin gene.

  title={Hepatocyte nuclear factor-1α regulates transcription of the guanylin gene.},
  author={Jay A Hochman and Daniela Sciaky and T L Whitaker and John A. Hawkins and Mitchell B. Cohen},
  journal={American journal of physiology. Gastrointestinal and liver physiology},
  volume={273 4},
To study the molecular mechanisms controlling guanylin expression, we have cloned the mouse guanylin gene, including 2.7 kb of upstream sequence. We show that the first 133 base pairs (bp) of the upstream guanylin promoter are sufficient to drive near maximal (6-fold over basal) luciferase reporter gene expression in Caco-2 intestinal cells; at least 300 bp of upstream promoter are required for reporter gene expression in HT-29 intestinal cell lines. Using electromobility shift assays, we… CONTINUE READING


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