Hepatocyte function and extracellular matrix geometry: long‐term culture in a sandwich configuration

@article{Dunn1989HepatocyteFA,
  title={Hepatocyte function and extracellular matrix geometry: long‐term culture in a sandwich configuration},
  author={J. Dunn and M. Yarmush and H. G. Koebe and R. Tompkins},
  journal={The FASEB Journal},
  year={1989},
  volume={3},
  pages={174 - 177}
}
  • J. Dunn, M. Yarmush, +1 author R. Tompkins
  • Published 1989
  • Biology, Medicine
  • The FASEB Journal
  • Adult rat hepatocytes cultured in a collagen sandwich system maintained normal morphology and a physiological rate of albumin secretion for at least 42 days. Hepatocytes cultured on a single layer of collagen gel essentially ceased albumin secretion within 1 wk but could recover function with the overlay of a second layer of collagen gel. This culture configuration more closely mimics the hepatocytes' in vivo environment and provides a simple method for their long‐term maintenance.— Dunn, J. C… CONTINUE READING
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