Hepatocyte DNA replication: effect of nutrients and intermediary metabolites.

  title={Hepatocyte DNA replication: effect of nutrients and intermediary metabolites.},
  author={Joan A. McGowan and William E. Russell and Nancy L. R. Bucher},
  journal={Federation proceedings},
  volume={43 1},
Isolated adult rat liver parenchymal cells maintained in serum-free medium are stimulated by insulin and epidermal growth factor (EGF) to undergo DNA synthesis. Pyruvate, lactate, and, to a lesser extent, several other intermediary metabolites strikingly enhance DNA synthesis both under serum-free culture conditions and in the presence of dialyzed rat serum. High concentrations (2-50 mM) of these low-molecular-weight metabolites are necessary to produce optimal stimulation. Both alanine… CONTINUE READING

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