Hepatitis C virus subgenomic replicon requires an active NS3 RNA helicase.

@article{Lam2006HepatitisCV,
  title={Hepatitis C virus subgenomic replicon requires an active NS3 RNA helicase.},
  author={Angela Lam and David N. Frick},
  journal={Journal of virology},
  year={2006},
  volume={80 1},
  pages={404-11}
}
Mutations were introduced into the NS3 helicase region of a hepatitis C virus (HCV) Con1 subgenomic replicon to ascertain the role of the helicase in viral replication. One new replicon lacked two-thirds of the NS3 helicase (Deltahel), and six others contained one of the following six amino acid substitutions in NS3: R393A, F438A, T450I, E493K, W501A, and W501F. It has been previously reported that purified R393A, F438A, and W501A HCV helicase proteins do not unwind RNA but unwind DNA, bind RNA… CONTINUE READING

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