Hepatitis C virus molecular clones: from cDNA to infectious virus particles in cell culture.


There has been major progress in our understanding of hepatitis C virus (HCV) molecular virology in recent years. An essential prerequisite for this progress was the availability of functional molecular HCV clones, that serve as a starting point in order to establish cell culture systems. The first of these was the HCV replicon system, which used self-replicating subgenomic viral RNAs. However, these replicons only recapitulated the intracellular life cycle, and did not support production of infectious virus: this became possible with the identification of an HCV isolate that, for unknown reasons, replicates to very high levels in a human hepatoma cell line. Cells containing this genome release virus particles that are infectious in cell culture and in vivo. Without doubt, this system provides new possibilities for molecular studies of the HCV life cycle and the development of novel antiviral concepts.


Citations per Year

246 Citations

Semantic Scholar estimates that this publication has 246 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Bartenschlager2006HepatitisCV, title={Hepatitis C virus molecular clones: from cDNA to infectious virus particles in cell culture.}, author={Ralf Bartenschlager}, journal={Current opinion in microbiology}, year={2006}, volume={9 4}, pages={416-22} }