Hepatitis C virus NS5B polymerase exhibits distinct nucleotide requirements for initiation and elongation.

Abstract

The hepatitis C virus (HCV) NS5B protein is an RNA-dependent RNA polymerase (RdRp) essential for replication of the viral RNA genome. Purified NS5B has been reported to exhibit multiple activities in vitro. Using a synthetic heteropolymeric RNA template with dideoxycytidine at its 3'-end, we examined de novo initiation and primer extension in a system… (More)
DOI: 10.1074/jbc.M803094200

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@article{Ferrari2008HepatitisCV, title={Hepatitis C virus NS5B polymerase exhibits distinct nucleotide requirements for initiation and elongation.}, author={Eric Ferrari and Zhiqing He and Robert E Palermo and H M Huang}, journal={The Journal of biological chemistry}, year={2008}, volume={283 49}, pages={33893-901} }