Hepatic tumor spread of colorectal cancer in a laparoscopic animal model

Abstract

Background: We devised a standardized animal model to study the impact of laparoscopic colorectal surgery on intrahepatic tumor cell growth. Methods: The technique of laparoscopic surgery in the rat was extended by endoscopic inoculation of colon cancer cells (CC531) into the portal vein (1 × 104, 5 × 104, 1 × 105, 3 × 105, 5 × 105 cells/ml) of WAG/Rij rats (n= 25). As controls, five animals underwent laparotomy and open intraportal inoculation of 5 × 104 cells/ml. Results: Hepatic tumor growth occurred after inoculation of 5 × 104, 1 × 105, 3 × 105, and 5 × 105 cells/ml. Extrahepatic tumor and conflating hepatic tumor was observed after the inoculation of 1 × 105, 3 × 105, and 5 × 105 cells/ml. Concentrations of 5 × 104 cells/ml injected either laparoscopically or via an open technique led to single hepatic tumor nodules. No tumor growth was seen after inoculation of 1 × 104 cells/ml. Conclusions: Laparoscopic intraportal tumor cell inoculation is a feasible technique to create hepatic metastases. The inoculation of 5 × 104 CC531 cells leads to reliable cell growth that can be used to investigate the impact of various laparoscopic techniques on tumor spread.

DOI: 10.1007/s004640000159

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@article{Gutt2000HepaticTS, title={Hepatic tumor spread of colorectal cancer in a laparoscopic animal model}, author={C. N. Gutt and Z. G. Kim and T. Gessmann and M. Lorenz and V. Paolucci}, journal={Surgical Endoscopy}, year={2000}, volume={14}, pages={448-451} }