Hepatic stellate cells contain the functional estrogen receptor beta but not the estrogen receptor alpha in male and female rats.

Abstract

In an earlier study, we showed that estradiol (E2) inhibits proliferation and transformation in cultured rat hepatic stellate cells (HSCs) and that the actions of E2 are mediated through estrogen receptors (ERs). This study reports on an investigation of the cellular localization of ER subtypes ERalpha and ERbeta using immunohistochemistry in experimental fibrotic liver rats and of each ER subtype expression in cultured rat HSCs by evaluating the produced mRNA and protein. The results indicate that high levels of ERbeta expression and low or no levels of ERalpha expression were observed in normal and fibrotic livers and in quiescent and activated HSCs from both males and females. The specificity of E2-mediated antiapoptotic induction through the ERbeta was shown by dose-dependent inhibition by the pure ER antagonist ICI 182,780 in HSCs which were undergoing early apoptosis. These findings demonstrate for the first time that rat HSCs possess functional Erbeta, but not Eralpha, to respond directly to E2 exposure.

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@article{Zhou2001HepaticSC, title={Hepatic stellate cells contain the functional estrogen receptor beta but not the estrogen receptor alpha in male and female rats.}, author={Yanheng Zhou and Ichiro Shimizu and Guo-yuan Lu and Mina Itonaga and Yoshihito Okamura and Masayuki Shono and H Honda and Satoshi Inoue and Masami Muramatsu and Satoru Ito}, journal={Biochemical and biophysical research communications}, year={2001}, volume={286 5}, pages={1059-65} }