Hepatic metabolism of diallyl disulphide in rat and man

@article{Germain2003HepaticMO,
  title={Hepatic metabolism of diallyl disulphide in rat and man},
  author={Eric Germain and Jo{\"e}lle Chevalier and M. H. Siess and Caroline Teyssier},
  journal={Xenobiotica},
  year={2003},
  volume={33},
  pages={1185 - 1199}
}
1. The metabolism of diallyl disulphide was investigated in vitro with rat and human liver cell subfractions and ex vivo with an isolated perfused rat liver. 2. Diallyl disulphide was oxidized to diallylthiosulphinate by rat liver microsomes with an apparent Km = 0.86 ± 0.1 mM and an apparent Vmax = 0.47 ± 0.12 nmol min−1 mg−1 protein (mean ± SE). Both cytochrome P450 (CYP) and flavin-containing monooxygenases were involved, with CYP2B1/2 and CYP2E1 being the most active CYP enzymes. 3. In rat… 

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