Hepatic glycogen synthesis is highly sensitive to phosphorylase activity: evidence from metabolic control analysis.

@article{Aiston2001HepaticGS,
  title={Hepatic glycogen synthesis is highly sensitive to phosphorylase activity: evidence from metabolic control analysis.},
  author={Susan Aiston and Lynne Hampson and Anna Maria G{\'o}mez-Foix and Joan J. Guinovart and Loranne Agius},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 26},
  pages={23858-66}
}
We used metabolic control analysis to determine the flux control coefficient of phosphorylase on glycogen synthesis in hepatocytes by titration with a specific phosphorylase inhibitor (CP-91149) or by expression of muscle phosphorylase using recombinant adenovirus. The muscle isoform was used because it is catalytically active in the b-state. CP-91149 inactivated phosphorylase with sequential activation of glycogen synthase. It increased glycogen synthesis by 7-fold at 5 mm glucose and by 2… CONTINUE READING
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