Hepatic Golgi fractions resolved into membrane and content subfractions

  title={Hepatic Golgi fractions resolved into membrane and content subfractions},
  author={Kathryn E. Howell and George Emil Palade},
  journal={The Journal of Cell Biology},
  pages={822 - 832}
Golgi fractions isolated from rat liver homogenates have been resolved into membrane and content subfractions by treatment with 100 mM Na2CO3 pH 11.3. This procedure permitted extensive extraction of content proteins and lipoproteins, presumably because it caused an alteration of Golgi membranes that minimized the reformation of closed vesicles. The type and degree of contamination of the fractions was assessed by electron microscopy and biochemical assays. The membrane subfraction retained 15… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.


Publications citing this paper.
Showing 1-10 of 60 extracted citations

Organelle Proteomics

Methods in Molecular Biology™ • 2008
View 13 Excerpts
Highly Influenced


Publications referenced by this paper.
Showing 1-4 of 4 references

Presence ofadenylate cyclase activity in Golgi and other fractions from rat liver . 11 . Cytochemical localization within Golgi and ER membranes

J. J. M. Bergeron
J . Cell Biol . • 1976

Isolation of cell surface membrane fractions from mammalian cells and organs

A. B. Novikoff

Polyacrylamide gel electrophoresis of viral proteins

J. Meldolesi

Similar Papers

Loading similar papers…