Hepatic Golgi fractions resolved into membrane and content subfractions

@article{Howell1982HepaticGF,
  title={Hepatic Golgi fractions resolved into membrane and content subfractions},
  author={Kathryn E. Howell and George Emil Palade},
  journal={The Journal of Cell Biology},
  year={1982},
  volume={92},
  pages={822 - 832}
}
Golgi fractions isolated from rat liver homogenates have been resolved into membrane and content subfractions by treatment with 100 mM Na2CO3 pH 11.3. This procedure permitted extensive extraction of content proteins and lipoproteins, presumably because it caused an alteration of Golgi membranes that minimized the reformation of closed vesicles. The type and degree of contamination of the fractions was assessed by electron microscopy and biochemical assays. The membrane subfraction retained 15… CONTINUE READING

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