Heparinase treatment of RNA before quantitative real-time RT-PCR.

  title={Heparinase treatment of RNA before quantitative real-time RT-PCR.},
  author={Mary Lynn Johnson and Chainarong Navanukraw and Anna T. Grazul-Bilska and Lawrence P Reynolds and Dale A. Redmer},
  volume={35 6},
          1140-2, 1144
Vol. 35, No. 6 (2003) BioTechniques 1141 the ABI PRISM® 7000 and the TaqMan Universal PCR Master Mix protocols (Applied Biosystems). After discovering that the RNA from the thecal cells had vascular endothelial growth factor (VEGF) amplification and that RNA from granulosa cells had no VEGF amplification, the 18S rRNA amplification (TaqMan Pre-Developed Assay Reagents; Applied Biosystems), which is used as a positive control for eukaryotic gene expression and to normalize RNA concentrations… CONTINUE READING

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