Heparin and dermatan sulphate induced capacitation of frozen-thawed bull spermatozoa measured by merocyanine-540.

@article{Bergqvist2007HeparinAD,
  title={Heparin and dermatan sulphate induced capacitation of frozen-thawed bull spermatozoa measured by merocyanine-540.},
  author={A S Bergqvist and J. D. Ballester and Anders Johannisson and Nils Lundeheim and Heriberto Rodriguez-Martinez},
  journal={Zygote},
  year={2007},
  volume={15 3},
  pages={225-32}
}
Glycosaminoglycans (GAGs) are present in the oviduct in which the major part of sperm capacitation occurs. In this study we have tested how capacitation of frozen-thawed bull spermatozoa is effected by exposure to different GAGs detectable or possibly present in oviductal fluid; i.e. heparin, hyaluronan, heparan sulphate, dermatan sulphate and chondroitin sulphate. Following exposure of different duration, the spermatozoa were stained with either Chlortetracycline (CTC) or merocyanine-540 and… CONTINUE READING
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