Heparin, platelet aggregation, neutrophils, and cardiopulmonary bypass.

@article{Belcher2000HeparinPA,
  title={Heparin, platelet aggregation, neutrophils, and cardiopulmonary bypass.},
  author={P. Belcher and E. Muriithi and E. Milne and P. Wanikiat and D. Wheatley and R. Armstrong},
  journal={Thrombosis research},
  year={2000},
  volume={98 4},
  pages={
          249-56
        }
}
UNLABELLED Cardiopulmonary bypass (CPB) is associated with both neutrophil activation and failure of platelets to form large stable aggregates. We aimed to determine the effects of heparin and of neutrophil activation on platelet aggregation in whole blood. Fourteen patients undergoing routine aortocoronary bypass grafting and NSAID-free for over 7 days were studied before and after heparinisation, and at end-CPB. Whole blood, anticoagulated with rHirudin, was stirred for 3 minutes, and… Expand
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References

SHOWING 1-10 OF 23 REFERENCES
The platelet function defect of cardiopulmonary bypass.
TLDR
CPB resulted in a markedly deficient platelet reactivity in response to an in vivo wound, but no loss of the platelet surface GPIb-IX and GPIIb-IIIa complexes, and a minimal number of circulating degranulated platelets. Expand
Impaired platelet aggregation after cardiopulmonary bypass in man: enhancement of collagen-induced aggregation in whole blood and plasma by adrenaline ex vivo.
1. We tested the effect of intravenous adrenaline at 0.55-1.10 nmol min-1 kg-1 (for 3-8 min, at 7-10 min post bypass; n = 7) on both microaggregation in hirudinized whole blood, using plateletExpand
Heparin causes platelet dysfunction and induces fibrinolysis before cardiopulmonary bypass.
TLDR
Heparin, independent of cardiopulmonary bypass, causes both platelet dysfunction and increased fibrinolysis, and the use of an alternative anticoagulant or a lower dose of heparin in conjunction with heparIn-coated surfaces might improve the hemostatic balance during open heart operations. Expand
Cardiopulmonary bypass induces leukocyte-platelet adhesion.
TLDR
It is concluded that CPB, through increased platelet GMP-140 expression, causes formation of monocyte-platelet, and to a lesser extent, PMN-platelets conjugates. Expand
Macroaggregation of platelets in plasma, as distinct from microaggregation in whole blood (and plasma), as determined using optical aggregometry and platelet counting respectively, is specifically impaired following cardiopulmonary bypass in man.
We determined changes in platelet aggregability following cardiopulmonary bypass, using optical aggregometry to assess macroaggregation in platelet-rich plasma (PRP), and platelet counting to assessExpand
Investigation of platelet-neutrophil interactions in whole blood by flow cytometry.
TLDR
A simple flow cytometric method for assessing and investigating platelet interactions with neutrophils in small volumes of whole blood and may also provide a method for estimating platelet activation in whole blood. Expand
Platelet aggregation during cardiopulmonary bypass evaluated by a laser light-scattering method.
TLDR
Generation of medium and large aggregates after stimulation with adenosine diphosphate and collagen were significantly decreased with CPB, whereas, in spite of hemodilution, the quantity of the small aggregates was maintained at the elevated level. Expand
Platelet-leukocyte activation and modulation of adhesion receptors in pediatric patients with congenital heart disease undergoing cardiopulmonary bypass.
TLDR
It is concluded that in children with congenital heart disease cardiopulmonary bypass causes loss of Platelet adhesion receptors, activation of platelets, formation of platelet-leukocyte conjugates, and leukocyte activation, and these cellular changes may contribute to both the hemostatic and inflammatory complications associated with cardiopULmonary bypass. Expand
Original article: the influence of aspirin on the proaggregatory action of adrenaline after cardiopulmonary bypass in man.
TLDR
It is indicated that at normocalcaemia, adrenaline enhanced collagen-induced PA in aspirin-treated patients, despite impairment of PA, but only at a high concentration of collagen. Expand
Downregulation of human platelet reactivity by neutrophils. Participation of lipoxygenase derivatives and adhesive proteins.
TLDR
In vitro biochemical and functional evidence is provided for the thromboregulatory role of neutrophils and the multicellular aspect of hemostasis and thromBosis is emphasized. Expand
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