Heparan sulfate-protein interactions: therapeutic potential through structure-function insights

@article{Coombe2004HeparanSI,
  title={Heparan sulfate-protein interactions: therapeutic potential through structure-function insights},
  author={Deirdre Roma Coombe and Warren C. Kett},
  journal={Cellular and Molecular Life Sciences CMLS},
  year={2004},
  volume={62},
  pages={410-424}
}
  • D. R. Coombe, W. Kett
  • Published 2004
  • Chemistry, Medicine
  • Cellular and Molecular Life Sciences CMLS
Abstract.Heparin and the related glycosaminoglycan, heparan sulfate, bind a myriad of proteins. The structural diversity of heparin and heparan sulfates is enormous, but differences in the conformational flexibility of the monosaccharide constituents add extra complexity and may influence protein binding. Silencing genes for heparin/ heparan sulfate biosynthetic enzymes profoundly affects mammalian development. Thus, altering the structure of heparan sulfate chains can alter protein binding and… Expand
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TLDR
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TLDR
An overview of the different methods used to synthesize heparosan is given, and the pro and cons of each method are highlighted in respect to the synthesis of bioengineered heparin-like molecules. Expand
On the Specificity of Heparin/Heparan Sulfate Binding to Proteins. Anion-Binding Sites on Antithrombin and Thrombin Are Fundamentally Different
TLDR
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TLDR
Alternatives for the provision of heparin such as chemical synthesized he parin, chemoenzymatic hepar in, and bioengineered heparins are discussed and the usage of other chromatographic systems mimetic the heparIn effect is reviewed. Expand
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TLDR
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Molecular Interactions between Complement Factor H and Its Heparin and Heparan Sulfate Ligands
TLDR
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Downstream Products are Potent Inhibitors of the Heparan Sulfate 2-O-Sulfotransferase
TLDR
The unexpected finding that oligosaccharides associated with later stages in HS biosynthesis inhibit HS2ST indicates that the enzyme must be separated temporally and/or spatially from downstream products during biosynthesis in vivo, and highlights a challenge for the enzymatic synthesis of lengthy HS chains in vitro. Expand
The Solution Structure of Heparan Sulfate Differs from That of Heparin
TLDR
The solution structures for eight purified HS fragments degree of polymerization 6–18 and dp24, corresponding to the predominantly unsulfated GlcA-GlcNAc domains of heparan sulfate, are analyzed and it is concluded that HS displays an extended bent conformation that is significantly distinct from that for heparin. Expand
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