Hemoglobin switching in unicellular erythroid culture of sibling erythroid burst-forming units: kit ligand induces a dose-dependent fetal hemoglobin reactivation potentiated by sodium butyrate.

@article{Gabbianelli2000HemoglobinSI,
  title={Hemoglobin switching in unicellular erythroid culture of sibling erythroid burst-forming units: kit ligand induces a dose-dependent fetal hemoglobin reactivation potentiated by sodium butyrate.},
  author={Marco Gabbianelli and Germana Castelli and Adriana Massa and Elvira Pelosi and Nadia Maria Sposi and Roberta Riccioni and Luisella Luchetti and Cesare Peschle},
  journal={Blood},
  year={2000},
  volume={95 11},
  pages={3555-61}
}
Mechanisms underlying fetal hemoglobin (HbF) reactivation in adult life have not been elucidated; particularly, the role of growth factors (GFs) is controversial. Interestingly, histone deacetylase (HD) inhibitors (sodium butyrate, NaB, trichostatin A, TSA) reactivate HbF. We developed a novel model system to investigate HbF reactivation: (1) single hematopoietic progenitor cells (HPCs) were seeded in serum-free unilineage erythroid culture; (2) the 4 daughter cells (erythroid burst-forming… CONTINUE READING