Hemoglobin loss from erythrocytes in vivo results from spleen-facilitated vesiculation.

@article{Willekens2003HemoglobinLF,
  title={Hemoglobin loss from erythrocytes in vivo results from spleen-facilitated vesiculation.},
  author={Frans L. A. Willekens and Bregt Roerdinkholder-Stoelwinder and Yvonne A. M. Groenen-D{\"o}pp and Harry J. Bos and Giel Bosman and Annegeet G van den Bos and Arie J. Verkleij and Jan M Werre},
  journal={Blood},
  year={2003},
  volume={101 2},
  pages={
          747-51
        }
}
Previous studies have shown that approximately 20% of hemoglobin is lost from circulating red blood cells (RBCs), mainly during the second half of the cells' life span. Because hemoglobin-containing vesicles are known to circulate in plasma, these vesicles were isolated. Flow cytometry studies showed that most RBC-derived vesicles contain hemoglobin with all hemoglobin components present. The hemoglobin composition of the vesicles resembled that of old RBCs. RBC cohort studies using isotope… 
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