Hemoglobin Determined in 15 1 aLof Whole Blood by “ Front - Face ” Fluorometry


We describe the rapid, inexpensive fluorometry of hemoglobin in undiluted whole blood. The procedure consists only of adding a standard quantity of a fluorescent dye to a measured volume (‘-‘15 .tL) of blood, together with some solubilizing detergent. The assay is based on the attenuation of the dye’s fluorescence (excited within the region 400-440 nm) that… (More)


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