Hemin-mediated oxidative inactivation of 3-hydroxy-3-methylglutaryl CoA reductase

@article{UshaDevi2004HeminmediatedOI,
  title={Hemin-mediated oxidative inactivation of 3-hydroxy-3-methylglutaryl CoA reductase},
  author={S. Usha Devi and Tangirala Ramasarma},
  journal={Molecular and Cellular Biochemistry},
  year={2004},
  volume={77},
  pages={103-110}
}
SummaryAddition of hemoglobin, methemoglobin, hemin or hematin in the assay mixture of rat liver 3-hydroxy-3-methylglutaryl CoA (HMGCoA) reductase inhibited the activity of the enzyme. The inhibition by hemin was rapid, without any apparent dependence on time of preincubation. At 20 μM hemin, a maximum of about 50% inhibition was obtained in the case of the microsomal enzyme while the solubilized enzyme showed almost 80%6 inhibition. Dithiothreitol at high concentrations or either of the two… 
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