Hemin-mediated oxidation of dithiothreitol reduces oxygen to H2O

  title={Hemin-mediated oxidation of dithiothreitol reduces oxygen to H2O},
  author={S. Usha Devi and Tangirala Ramasarma},
  journal={Molecular and Cellular Biochemistry},
SummaryHemin catalyses the oxidation of dithiothreitol. One mole of oxygen is consumed for every 2 moles of dithiothreitol oxidized and the product is shown by spectral studies to be the intramolecular disulphide. The reaction shows a specificity for dithiol and for free heme moieties. Hemin molecules exhibit cooperativity in oxygen reduction. Oxygen radicals do not seem to be involved. H2O2 is not required for this oxidation of dithiothreitol and does not appear to be an intermediate in the… 

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